Ecology and Evolution of microorganisms (EMI-E0339)

Odile BOUVET
Research scientist
email: odile.bouvet@inserm.fr

Inserm U722

Ecology and evolution of microorganisms

Contact information - Inserm U722
Faculté de Médecine Xavier Bichat
16, rue Henri Huchard -- 75018 Paris (France)

Tel: 33 (0)1 57 27 77 42 -- Fax: 33 (0)1 57 27 73 72
Bureau: 396

Research interest

Education

List of the 10 main publications

Exhaustive list of publications

Research interest

Adaptation to environmental stresses is essential for the survival of all organisms. They almost invariably respond to changes in their environment by modifications in genetic constitution or phenotypic adaptation. In order to study the phenotypic polymorphism in commensal and pathogenic Escherichia coli isolates, the metabolic and physiological responses of strains grown in different conditions are studied. The capacities of colonization and pathogenesis of bacteria depend on their genotypic characteristics but also on their physiological characteristics. The whole-genome sequences of E. coli strains provide an opportunity to study phenotype-genotype correlations and to begin to understand how intraspecies physiologic diversity impacts host-pathogen interactions.

Education

Degrees
2000:	Diplôme d’Habilitation à Diriger des Recherches
1989:	Ph. D. in Microbiology, Paris VII University
1985:	Diplôme de Bactériologie Générale, Institut Pasteur Paris
1984:	M. Sc in Microbiology, Paris VII University
1982:	Diplôme de Bactériologie et de Virologie Systématiques, Institut Pasteur Paris
1981:	CES Immunologie Générale, Paris VI University
1980:	Resident, University Hospitals of Paris
1979:	Pharmacy diploma (option biologie), Paris VI University
Academic positions
2005:	Research position in Inserm U722, Ecology and Evolution of Microorganisms
2002-2004:	Research position in Inserm U550, Subject: "Environmental signals implicated in survival and virulence of Afa/Dr diffusely adhering Escherichia coli" (University of Paris XI)
1993-2001:	Head of the team "Metabolic diversity in Bacteria" at the Inserm unit 389, Institut Pasteur, Paris
1989-1992:	Research position in Inserm unit 199, Institut Pasteur, Paris
Others
1989-present:	Bacteriology course, Institut Pasteur Paris
1995-2000:	Bacteriology and Mycology dept, Institut Pasteur, Paris, Concil member

List of the 10 main publications

(* Indicates the authors who have equally contributed to the work)

	DIARD S., LIEVIN-LE MOAL V., TORIBIO A.L., BOUM Y., VIGIER F., SERVIN A.L., BOUVET O. Norepinephrine-dependently released Dr fimbriae of diffusely adhering Escherichia coli strain IH11128 promotes a mitogen-activated protein kinase ERK1/2-dependent production of pro-inflammatory cytokine, IL-8 in human intestinal Caco-2/TC7 cells. Microbes Infect., 2009, 11, 886-894
	DIARD S., TORIBIO A.L., BOUM Y., VIGIER F., KANSAU K., BOUVET O., SERVIN A. *Environmental signals implicated in Dr fimbriae release of pathogenic Escherichia* coli.** Microbes Infect., 2006, 8, 1851-1858
	VILLARINO A., RAGER M-N., GRIMONT PAD., AND BOUVET O. *Are UV-induced non-culturable Escherichia* coli K-12 cells alive or dead ?** Eur. J. Biochem. 27., 2003, 2689-2695
	NASSIF N., BOUVET O., RAGER M-N., ROUX C., CORADIN T., LIVAGE J. Living bacteria in silica gels Nature material., 2002, 1, 42-44
	DIARD S., CARLIER JP., AGERON E., GRIMONT PAD., LANGLOIS V., GUERIN P. AND BOUVET O. Accumulation of poly(3-hydroxybutyrate) from octanoate in different Pseudomonas belonging to the rRNA homology group 1. Systematic and Applied Microbiolog., 2002, 25, 183-188
	RAGER M.N., MARIE R.B. BINET, IONESCU G, BOUVET O. 31P and 13C Nuclear Magnetic Resonance Studies of Mannose Metabolism in Plesiomonas shigelloides : Toxic effect of mannose on growth. Eur. J. Bioche., 2000, 267, 5136-41.
	RAGER M.N., BINET M.R.AND BOUVET O. 31P and 13C Nuclear Magnetic Resonance studies of metabolic pathways in Pasteurella multocida : characterization of a new mannitol-producing metabolic pathway. Eur. J. Biochem, 1999, 263, 1-8.
	BINET M.R.B., BOUVET O. Fructose and mannose metabolism inAeromonas hydrophila : identification of transport systems and catabolic pathways. Microbiology, 1998, 144, 1113-1121.
	DE CRECY - LAGARD V., LEJEUNE P., BOUVET O., DANCHIN A. Identification of two fructose permeation and phosphorylation pathways in Xanthomonas campestris pv. campestris. Mol. Gen. Genet, 1991, 222:465-472.
	DE CRECY - LAGARD V., BOUVET O., LEJEUNE P., DANCHIN A. Fructose catabolism in Xanthomonas campestris pv. campestris. J. Biol. Chem,1991, 266 :18154-18161.

Exhaustive list of publications

(* Indicates the authors who have equally contributed to the work)

	AUBRON C., HUET O., RICOME S., BORDERIE D., PUSSARD E., LEBLANC P.E., BOUVET O., VICAUT E., DENAMUR E., DURANTEAU J. Changes in urine composition after trauma facilitate bacterial growth. BMC Infect Dis, 2012,(In press)
	AUBRON C., GLODT J., MATAR C., HUET O., BORDERIE D., DOBRINDT U., DURANTEAU J., DENAMUR E., CONTI M., BOUVET O. Variation in endogenous oxidative stress in Escherichia coli natural isolates during growth in urine. BMC Microbiology, 2012, 12, 120
	VIEIRA G., SABARLY V., BOURGUIGNON P.Y., DUROT M., LE FÈVRE F., MORNICO D., VALLENET D., BOUVET O., DENAMUR E., SCHACHTER V., MÉDIGUE C. The core and pan-metabolism in the Escherichia coli species. J. Bacteriol., 2011, 193, 1461-1472
	SABARLY V., BOUVET O., GLODT J., CLERMONT O., SKURNIK D., BRISSE S., DE VIENNE D., DENAMUR E., DILLMANN C. The decoupling between genetic structure and metabolic phenotypes in Escherichia coli leads to continuous phenotypic diversity. J. Evol. Biol., 2011, 24, 1559-1571
	LEVERT M., ZAMFIR O., CLERMONT O., BOUVET O., LESPINATS S., HIPEAUX M.C., BRANGER C., PICARD B., SAINT-RUF C., NOREL F., BALLIAU T., ZIVY M., LE NAGARD H., CRUVELLIER S., CHANE-WOON-MING B., NILSSON S., GUDELJ I., PHAN K., FERENCI T., TENAILLON O., DENAMUR E. Molecular and evolutionary bases of within-patient genotypic and phenotypic diversity in Escherichia coli extraintestinal infections. PLoS Pathog., 2010, 6, e1001125
	TOUCHON T., HOEDE H., TENAILLON O., BARBE V., BAERISWYL S., BIDET P., BINGEN E., BONACORSI S., BOUCHIER C., BOUVET O., CALTEAU A., CHIAPELLO H., CLERMONT O., CRUVEILLER S., DANCHIN A., DIARD M., DOSSAT C., EL KAROUI M., FRAPY E., GARRY L., GHIGO J.M., GILLES A.M., JOHNSON J.R., LE BOUGUENEC C., LESCAT M., MANGENOT S., MARTINEZ-JEHANNE V., MATIC I., NASSIF S., OZTAS S., PETIT M.A., PICHON C., ROUY Z., SAINT RUF C., SCHNEIDER D., TOURRET J., VACHERIE B., VALLENET D., MEDIGUE C., ROCHA E., DENAMUR E. Organised genome dynamics in the Escherichia* coli species results in highly diverse adaptive paths. PLoS Genet., 2009, 5, e1000344
	DIARD S., LIEVIN-LE MOAL V., TORIBIO A.L., BOUM Y., VIGIER F., SERVIN A.L., BOUVET O. Norepinephrine-dependently released Dr fimbriae of diffusely adhering Escherichia coli strain IH11128 promotes a mitogen-activated protein kinase ERK1/2-dependent production of pro-inflammatory cytokine, IL-8 in human intestinal Caco-2/TC7 cells. Microbes Infect., 2009, 11, 886-894
	DESMARD M., DAVIDGE K., BOUVET O., MORIN D., ROUX D., FORESTI R., RICARD J.D., DENAMUR E., POOPLE R.K., MONTRAVERS P., MOTTERLINI R., BOCZKOWSKI J. A carbon monoxyde-releasing molecule (CORM-3) exerts bactericidal activity against Pseudomonas aeruginosa and improves survival in an animal model of bacteraemia, FASEB J., 2009, 23, 1023-1031
	DIARD S., TORIBIO A.L., BOUM Y., VIGIER F., KANSAU K., BOUVET O., SERVIN A. Environmental signals implicated in Dr fimbriae release of pathogenic Escherichia coli. Microbes Infect., 2006, 8, 1851-1858
	NASSIF N., ROUX C., CORADIN T., BOUVET O., LIVAGE J. Bacteria quorum sensing in silica matrices. Journal of Materials Chemistry 1., 2004, 2264-2268.
	BIVILLE F., OSHIMA T., MORI HI., KAWAGOE Y., BOUVET O., RAGER M-N, PERROTTE-PIQUEMAL M., AND DANCHIN A. Escherichia coli response to exogenous pyrophosphate and analogs. J Mol Microbiol Biotechnol. ., 2003, 37-45
	NASSIF N., ROUX C., T. CORADIN T., LORENTE G., RAGER M-N., BOUVET O., LIVAGE J. A Sol-Gel Matrix to Preserve the Viability of Encapsulated Bacteria. J. Mater. Che., 2003, 13, 203-208
	VILLARINO A., RAGER M-N., GRIMONT PAD., AND BOUVET O. Are UV-induced non-culturable Escherichia coli K-12 cells alive or dead ? Eur. J. Biochem. 27., 2003, 2689-2695
	VILLARINO A., A., TORIBIO L., BRENA BM., GRIMONT PAD AND BOUVET O. On the relationship between the physiological state of bacteria and rapid enzymatic assays of fecal coliforms in the environment. Biotech Lett 2., 2003, 1329-1334
	NASSIF N., COIFFIER A., CORADIN T., ROUX C., BOUVET O., LIVAGE J. Viability of bacteria in hybrid aqueous silica gels. J. Sol-Gel Sci Technol., 2002, 26, 1-4
	DIARD S., CARLIER JP., AGERON E., GRIMONT PAD., LANGLOIS V., GUERIN P. AND BOUVET O. Accumulation of poly(3-hydroxybutyrate) from octanoate in different Pseudomonas belonging to the rRNA homology group 1. Systematic and Applied Microbiolog., 2002, 25, 183-188
	NASSIF N., BOUVET O., RAGER M-N., ROUX C., CORADIN T., LIVAGE J. Living bacteria in silica gels Nature material., 2002, 1, 42-44
	COIFFIER A., CORADIN T., ROUX C., BOUVET O., LIVAGE J. Sol-gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes. J. Mater. Che., 2001, 11, 2039-2044
	LIVAGE L., ROUX C., CORADIN T., FENNOUTH S., GUYON S., BERGOGNE L., COIFFIER A., . BOUVET O. Enzymes and cells confined in silica nanopores. Mater. Res. Soc. Symp. Pro., 2001, 651, 1-10.
	VILLARINO A., BOUVET O., REGNAULT B., DELAUTRE S.AND GRIMONT PAD Cellular activities in ultra-violet killed Escherichia coli. Int. J. Food Microbiol., 2000, 55, 245-247.
	RAGER M.N., MARIE R.B. BINET, IONESCU G, BOUVET O. 31P and 13C Nuclear Magnetic Resonance Studies of Mannose Metabolism in Plesiomonas shigelloides : Toxic effect of mannose on growth. Eur. J. Bioche., 2000, 267, 5136-41.
	VILLARINO A., BOUVET O., REGNAULT B., DELAUTRE S. AND GRIMONT PAD. Exploring the frontier between life and death in bacteria : evaluation of different viability markers in live, heat and UV-killed Escherichia coli cells. Res. Microbiol., 2000, 151, 755-768.
	BEAR M., LANGLOIS V., ROBIC D., BOURBOUZE R., BOUVET O. AND GUERIN P. Natural and artificial functionalized biopolyesters. Medium-length chain polyhydroxyoctanoates from pseudomonas strains. J. Env. Polym. Degrad 7(4., 2000, 179-184.
	BOUVET O. AND RAGER M-N. Sugar transport and metabolism in fermentative bacteria. In “NMR and Microbiology”. JN Barbotin and JC Portais, Eds., 2000, Chapter 16, pp 349-361 Horizon Scientific Press, UK.
	RAGER M.N., BINET M.R.AND BOUVET O. 31P and 13C Nuclear Magnetic Resonance studies of metabolic pathways in Pasteurella multocida : characterization of a new mannitol-producing metabolic pathway. Eur. J. Biochem, 1999, 263, 1-8.
	BOUVET O., PERNOUD S. AND GRIMONT PAD. Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7. Appl. Environ. Microbiol. , 1999, 65 -4245-4247.
	BINET M.R.B., BOUVET O. Fructose and mannose metabolism inAeromonas hydrophila : identification of transport systems and catabolic pathways. Microbiology, 1998, 144, 1113-1121.
	BINET M.R.B., BOUVET O. Transport of glucose by a phosphoenolpyruvate : mannose phosphotransferase system in Pasteurella multocida. Res. Microbiol., 1997, 149, 83-94.
	RIEU LESME F., DAUGA C., BOUVET O., GRIMONT PAD.AND J. DORE. Acetogenic coccoid spore-forming bacteria isolated from the rumen. Res. Microbiol., 1996, 147 :753-764.
	BOUVET O., LENORMAND P., E. AGERON E.AND GRIMONT PAD. Taxonomic diversity of the anaerobic glycerol dissimilation in the Enterobacteriaceae. Res. Microbiol., 1995, 146 :279-290.
	BOUVET O., LENORMAND P. AND GRIMONT PAD. Differentiation of Shigella species from Escherichia coli by glycerol dehydrogenase activity Res. Microbiol., 1995, 146 :787-790.
	BOUVET O., LENORMAND P., CARLIER J.P. AND GRIMONT PAD. Phenotypic diversity of anaerobic glycerol dissimilation shown by seven enterobacterial species. Res. Microbiol., 1994, 145 :129-139.
	DE CRECY - LAGARD V., LEJEUNE P., BOUVET O., DANCHIN A. Identification of two fructose permeation and phosphorylation pathways in Xanthomonas campestris pv. campestris. Mol. Gen. Genet, 1991, 222:465-472.
	DE CRECY - LAGARD V., BOUVET O., LEJEUNE P., DANCHIN A. Fructose catabolism in Xanthomonas campestris pv. campestris. J. Biol. Chem,1991, 266 :18154-18161.
	BOUVET O., LENORMAND P, GRIMONT PAD. Taxonomic diversity of the D-glucose oxidation pathway in the Enterobacteriaceae. Int. J. Syst. Bacteriol., 1989, 39:61-67.
	GRIMONT PAD., BOUVET O. Diversity of glucose entry route in Enterobacteriaceae. FEMS Microbiol. Reviews,1989, 63:109-114.
	BOUVET PJM., BOUVET O. Glucose dehydrogenase activity in Acinetobacter species. Res. Microbiol., 1989, 140:531-540.
	BOUVET O., GRIMONT PAD. Extracellular oxidation of D-glucose by some members of the Enterobacteriaceae. Ann. Inst. Pasteur/Microbiol.,1988, 139:59-77.
	BOUVET O., GRIMONT PAD. Evolutionary divergence of enterobacterial genes coding for the glucose phosphotransferase system components. FEMS Microbiol. Letters, 1988, 56:145-150.
	BOUVET O., GRIMONT PAD. Diversity of the phosphoenolpyruvate glucose phosphotransferase system in the Enterobacteriaceae. Ann.Inst.Pasteur/Microbiol. 1987, 138:3-13.
	BOUVET O., GRIMONT PAD. , RICHARD C., ALDOVA E., AUSNER O., GABRHELOVA M. Budvicia aquatica gen. nov., sp.nov. : a hydrogen sulfide-producing memberof the Enterobacteriaceae. Int. J. Syst. Bacteriol.,1985, 35:60-64.