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Odile Bouvet
Research scientist
email :odile.bouvet@inserm.fr

Inserm U722

Ecology and evolution of microorganisms



Contact information - Inserm U722
Faculté de Médecine Xavier Bichat
16, rue Henri Huchard -- 75018 Paris (France)

Tel: 33 (0)1 57 27 77 42 -- Fax: 33 (0)1 57 27 77 24
3ème étage - ascenseur B - pièce 396




Research interest


Adaptation to environmental stresses is essential for the survival of all organisms. They almost invariably respond to changes in their environment by modifications in genetic constitution or phenotypic adaptation. In order to study the phenotypic polymorphism in commensal and pathogenic Escherichia coli isolates, the metabolic and physiological responses (fluxome) of strains grown in different conditions are studied using NMR together with the measurements of enzyme activities and intracellular metabolite concentrations (metabolome). The capacities of colonization and pathogenesis of bacteria depend on their genotypic characteristics but also on their physiological characteristics. The whole-genome sequences of E. coli strains provide an opportunity to study phenotype-genotype correlations and to begin to understand how intraspecies physiologic diversity impacts host-pathogen interactions.

Education

Degrees

2000
 Diplôme d’Habilitation à Diriger des Recherches
1989
 Ph. D. in Microbiology, Paris VII University
1985
 Diplôme de Bactériologie Générale, Institut Pasteur Paris
1984
 M. Sc in Microbiology, Paris VII University
1982
 Diplôme de Bactériologie et de Virologie Systématiques, Institut Pasteur Paris
1981
 CES Immunologie Générale, Paris VI University
1980
 Resident, University Hospitals of Paris
1979
 Pharmacy diploma (option biologie), Paris VI University

Academic positions

2005
 Research position in Inserm U722, Ecology and Evolution of Microorganisms
2002-2004
 Research position in Inserm U550, Subject: "Environmental signals implicated in survival and virulence of Afa/Dr diffusely adhering Escherichia coli" (University of Paris XI)
1993-2001
 Head of the team "Metabolic diversity in Bacteria" at the Inserm unit 389, Institut Pasteur, Paris
1989-1992
 Research position in Inserm unit 199, Institut Pasteur, Paris

Bacteriology course, Institut Pasteur Paris (1989-present)
Bacteriology and Mycology dept, Institut Pasteur, Paris, Concil member 1995-2000

List of the 10 main publications
DE CRECY-LAGARD V., LEJEUNE P., BOUVET OM, DANCHIN A.
Identification of two fructose permeation and phosphorylation pathways in Xanthomonas campestris pv. campestris. Mol. Gen. Genet, 1991, 222:465-472.
DE CRECY-LAGARD V., BOUVET OM., LEJEUNE P., DANCHIN A.
Fructose catabolism in Xanthomonas campestris pv. campestris. J. Biol. Chem,1991, 266 :18154-18161.
BINET M.R., BOUVET OM. .
Fructose and mannose metabolism inAeromonas hydrophila : identification of transport systems and catabolic pathways. Microbiology, 1998, 144, 1113-1121.
RAGER M.N., M.R.B. BINET and BOUVET OM.
31P and 13C Nuclear Magnetic Resonance studies of metabolic pathways in Pasteurella multocida : characterization of a new mannitol-producing metabolic pathway. Eur. J. Biochem, 1999, 263, 1-8.
BOUVET O.M., PERNOUD S.and GRIMONT PAD.
Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7. Appl. Environ. Microbiol. , 1999, 65 -4245-4247.
RAGER M.N., BINET M.R.B., IONESCU G., BOUVET OM, 2000,
31P and 13C Nuclear Magnetic Resonance Studies of Mannose Metabolism in Plesiomonas shigelloides : Toxic effect of mannose on growth. Eur. J. Biochem, 267, 5136-41.
DIARD S., CARLIER JP., AGERON E., GRIMONT PAD., LANGLOIS V., GUERIN P. and BOUVET OM, 2002,
Accumulation of poly(3-hydroxybutyrate) from octanoate in different Pseudomonas belonging to the rRNA homology group 1. Systematic and Applied Microbiology, 25, 183-188
NASSIF N., BOUVET OM, RAGER M-N, ROUX C., CORADIN T., LIVAGE J., 2002,
Living bacteria in silica gels, Nature materials, 1, 42-44
NASSIF N., ROUX C., CORADIN C., LORENTE G., RAGER M-N, BOUVET OM, LIVAGE J., 2003,
A Sol-Gel Matrix to Preserve the Viability of Encapsulated Bacteria, J. Mater. Chem, 13, 203-208
VILLARINO A., RAGER M-N, GRIMONT PAD. , and BOUVET OM., 2003,
Are UV-induced non-culturable Escherichia coli K-12 cells alive or dead ? Eur. J. Biochem. 270, 2689-2695
Exhaustive list of publications
DIARD S., TORIBIO A.L., BOUM Y., VIGIER F., KANSAU K., BOUVET O., SERVIN A.
Environmental signals implicated in Dr fimbriae release of pathogenic Escherichia coli.
Microbes Infect., 2006, 8, 1851-1858
BOUVET OMM., GRIMONT PAD. , RICHARD C., ALDOVA E., AUSNER O., GABRHELOVA M.
Budvicia aquatica gen. nov., sp.nov. : a hydrogen sulfide-producing memberof the Enterobacteriaceae. Int. J. Syst. Bacteriol.,1985, 35:60-64.
BOUVET OMM., GRIMONT PAD.
Diversity of the phosphoenolpyruvate glucose phosphotransferase system in the Enterobacteriaceae. Ann.Inst.Pasteur/Microbiol. 1987, 138:3-13.
BOUVET OMM., GRIMONT PAD.
Extracellular oxidation of D-glucose by some members of the Enterobacteriaceae. Ann. Inst. Pasteur/Microbiol.,1988, 139:59-77.
BOUVET OMM., GRIMONT PAD.
Evolutionary divergence of enterobacterial genes coding for the glucose phosphotransferase system components. FEMS Microbiol. Letters, 1988, 56:145-150.
BOUVET OMM., LENORMAND P, GRIMONT PAD.
Taxonomic diversity of the D-glucose oxidation pathway in the Enterobacteriaceae. Int. J. Syst. Bacteriol., 1989, 39:61-67.
GRIMONT PAD., BOUVET OMM.
Diversity of glucose entry route inEnterobacteriaceae. FEMS Microbiol. Reviews,1989, 63:109-114.
BOUVET PJM., BOUVET OMM.
Glucose dehydrogenase activity in Acinetobacter species. Res. Microbiol., 1989, 140:531-540.
DE CRECY - LAGARD V., LEJEUNE P., BOUVET OMM, DANCHIN A.
Identification of two fructose permeation and phosphorylation pathways in Xanthomonas campestris pv. campestris.. Mol. Gen. Genet, 1991, 222:465-472.
DE CRECY - LAGARD V., BOUVET OMM., LEJEUNE P., DANCHIN A..
Fructose catabolism in Xanthomonas campestris pv. campestris. J. Biol. Chem,1991, 266 :18154-18161.
BOUVET OMM., LENORMAND P., CARLIER J.P. and GRIMONT PAD.
Phenotypic diversity of anaerobic glycerol dissimilation shown by seven enterobacterial species. Res. Microbiol., 1994, 145 :129-139.
BOUVET OMM., LENORMAND P., E. AGERON E.and GRIMONT PAD.
Taxonomic diversity of the anaerobic glycerol dissimilation in the Enterobacteriaceae. Res. Microbiol., 1995, 146 :279-290.
BOUVET OMM., LENORMAND P. and GRIMONT PAD.
Differentiation of Shigella species from Escherichia coli by glycerol dehydrogenase activity Res. Microbiol., 1995, 146 :787-790.
RIEU LESME F., DAUGA C., BOUVET OMM., GRIMONT PAD.and J. DORE.
Acetogenic coccoid spore-forming bacteria isolated from the rumen. Res. Microbiol., 1996, 147 :753-764.
BINET M.R.B., BOUVET OMM.
Transport of glucose by a phosphoenolpyruvate : mannose phosphotransferase system in Pasteurella multocida. Res. Microbiol., 1997, 149, 83-94.
BINET M.R.B., BOUVET OMM.
Fructose and mannose metabolism inAeromonas hydrophila : identification of transport systems and catabolic pathways. Microbiology, 1998, 144, 1113-1121.
RAGER M.N., BINET M.R.and BOUVET OMM.
31P and 13C Nuclear Magnetic Resonance studies of metabolic pathways in Pasteurella multocida : characterization of a new mannitol-producing metabolic pathway. Eur. J. Biochem, 1999, 263, 1-8.
BOUVET OMM., PERNOUD S. and GRIMONT PAD..
Temperature-dependent fermentation of D-sorbitol in Escherichia coli O157:H7. Appl. Environ. Microbiol. , 1999, 65 -4245-4247.
VILLARINO A., BOUVET OMM., REGNAULT B., DELAUTRE S.and GRIMONT PAD, 2000,
Cellular activities in ultra-violet killed Escherichia coli. Int. J. Food Microbiol., 55, 245-247.
Marie Noelle RAGER, Marie R.B. BINET, Gabriel IONESCU, Odile M.M.BOUVET, 2000,
31P and 13C Nuclear Magnetic Resonance Studies of Mannose Metabolism in Plesiomonas shigelloides : Toxic effect of mannose on growth. Eur. J. Biochem, 267, 5136-41.
VILLARINO A., BOUVET OMM., REGNAULT B., DELAUTRE S. and GRIMONT PAD., 2000,
Exploring the frontier between life and death in bacteria : evaluation of different viability markers in live, heat and UV-killed Escherichia coli cells. Res. Microbiol., 151, 755-768.
BEAR M., LANGLOIS V., ROBIC D., BOURBOUZE R., BOUVET OMM. and GUERIN P., 2000,
Natural and artificial functionalized biopolyesters. Medium-length chain polyhydroxyoctanoates from pseudomonas strains. J. Env. Polym. Degrad 7(4), 179-184.
BOUVET OMM. and RAGER M-N.
Sugar transport and metabolism in fermentative bacteria. In “NMR and Microbiology”. JN Barbotin and JC Portais, Eds. 2000, Chapter 16, pp 349-361 Horizon Scientific Press, UK.
COIFFIER A., CORADIN T., ROUX C., BOUVET OMM., LIVAGE J., 2001,
Sol-gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes, J. Mater. Chem, 11, 2039-2044
LIVAGE L., ROUX C., CORADIN T., FENNOUTH S., GUYON S., BERGOGNE L., COIFFIER A., . BOUVET OMM., 2001,
Enzymes and cells confined in silica nanopores. Mater. Res. Soc. Symp. Proc, 651, 1-10.
NASSIF N., COIFFIER A., CORADIN T., ROUX C., BOUVET OMM., LIVAGE J., 2002,
Viability of bacteria in hybrid aqueous silica gels. J. Sol-Gel Sci Technol., 26, 1-4

DIARD S., CARLIER JP., AGERON E., GRIMONT PAD., LANGLOIS V., GUERIN P. and BOUVET OMM., 2002,
Accumulation of poly(3-hydroxybutyrate) from octanoate in different Pseudomonas belonging to the rRNA homology group 1. Systematic and Applied Microbiology, 25, 183-188
NASSIF N., BOUVET O., RAGER M-N., ROUX C., CORADIN T., LIVAGE J., 2002,
Living bacteria in silica gels, Nature materials, 1, 42-44
BIVILLE F., OSHIMA T., MORI HI., KAWAGOE Y., BOUVET O., RAGER M-N, PERROTTE-PIQUEMAL M., and DANCHIN A., 2003,
Escherichia coli response to exogenous pyrophosphate and analogs. J Mol Microbiol Biotechnol. 1, 37-45
NASSIF N., ROUX C., T. CORADIN T., LORENTE G., RAGER M-N., BOUVET O., LIVAGE J., 2003,
A Sol-Gel Matrix to Preserve the Viability of Encapsulated Bacteria, J. Mater. Chem, 13, 203-208
VILLARINO A., RAGER M-N., GRIMONT PAD., and BOUVET OMM., 2003,
Are UV-induced non-culturable Escherichia coli K-12 cells alive or dead ? Eur. J. Biochem. 270, 2689-2695
VILLARINO A., A., TORIBIO L., BRENA BM., GRIMONT PAD and BOUVET OMM. 2003.
On the relationship between the physiological state of bacteria and rapid enzymatic assays of fecal coliforms in the environment. Biotech Lett 25, 1329-1334
NASSIF N., ROUX C., CORADIN T., BOUVET O., LIVAGE J., 2004,
Bacteria quorum sensing in silica matrices. Journal of Materials Chemistry 14, 2264-2268.

CORADIN T., NASSIF N., ROUX C., GUISTINIANI J., ROUX C., BOUVET O., LIVAGE J.. 2004.
Bacteria in silica gels : how can we keep them alive ? Solid State Sciences (in press).